PreCursor-M AnoGYN

• Method: Multiplex real-time methylation-specific PCR assay
• Target: Human genes ASCL1 and ZNF582
• Built-in reference and sample quality control: β-actin (ACTB)
• Calibrator: Contains a standard concentration of low-copy DNA fragments of the two targets ASCL1, ZNF582 and the control gene ACTB – also used for run validation
• Sample types: Bisulfite-converted DNA prepared from human samples, for instance formalin-fixed paraffin embedded anal biopsies or anal swab specimens
• Sample volume: 5 μL of bisulfite-converted DNA
• Total time to result: Ranging between 2 to 24 hours depending on the level of automation (incl. DNA extraction and bisulfite conversion)
• Equipment: Mic qPCR cycler (Bio Molecular Systems), Rotor-Gene Q 5plex system (Qiagen) or ViiA 7 qPCR system (Thermo Fisher Scientific)

References:

1. Costello, J.F., and Plass C. (2001) Methylation matters. J. Med. Genet. 38, 285-303.
2. van der Zee R.P. , et al. Host cell deoxyribonucleic acid methylation markers for the detection of high-grade anal intraepithelial neoplasia and anal cancer. Clin. Infect. Dis., 68 (7); 1110-1117, 2019
3. van der Zee R.P. , et al. Cancer risk stratification of anal intraepithelial neoplasia in human immunodeficiency virus-positive men by validated methylation markers associated with progression to cancer. Clin. Infect. Dis., 72 (12); 2154-2163, 2021
4. van der Zee R.P. , et al. DNA methylation markers have universal prognostic value for anal cancer risk in HIV-negative and HIV-positive individuals. Mol. Oncol., 15 (11); 3024-3036, 2021, 2021
5. Rozemeijer K, et al. Analytical validation and diagnostic performance of the ASCL1/ZNF582 methylation test for detection of high-grade anal intraepithelial neoplasia and anal cancer. Tumour Virus Res. 30;17:200275, 2023
6. Steenbergen, R.D.M. et al. (2014) Clinical implications of (epi)genetic changes in HPV-induced precancerous lesions. Nat. Rev. Cancer 14, 395–405.

To read the end user conditions of sale for this product please visit our Resource center.